http://www.humanpathol.com/article/S0046-8177(14)00108-7/abstract?rss=yes
Osteoblastoma (OB) y osteosarcoma (OS) son 2 tumores óseos que afectan principalmente a los adultos jóvenes. El manejo clínico de OS difiere significativamente de la de OB, y por lo tanto, un diagnóstico preciso de OB y OS es crítico en la determinación de la modalidad de tratamiento apropiado. Sin embargo, en ciertos casos, el sistema operativo se superpone significativamente con OB en las características clínicas y radiográficas, y por lo tanto, el diagnóstico diferencial de OB y OS puede ser difícil, especialmente cuando el material de biopsia es insuficiente. Hasta la fecha, ha habido pocos informes sobre los marcadores para el diagnóstico diferencial de la OB y OS. Hemos demostrado anteriormente que el / β-catenina vía Wnt es inactivado en la SG. En este estudio, que tuvo como objetivo investigar si el patrón de distribución de celulares β-catenina es un marcador potencial para el diagnóstico diferencial de OB y OS. La tinción inmunohistoquímica se estudió en 17 muestras de OB (21 biopsias, 17 primarias y 4 recurrentes) y 37 muestras de OS con información de seguimiento completo. Moderada a fuerte tinción β-catenina nuclear se encontró en todos los especímenes de OB (17/17). En contraste, la tinción positiva de β-catenina se encuentra en el citoplasma y / o membrana, pero no el núcleo en todos los 32 casos de OS nonchondroblastic (32/32) y el componente clásico OS en OS condroblástico (5/5). La única tinción β-catenina nuclear positivo detectado en biopsias OS estaba en las células del sistema operativo condroblástico (5/5). En resumen, nuestros resultados indican que, además de la evaluación histopatológica convencional, la distribución celular de β-catenina puede ser utilizado como un marcador útil en el diagnóstico diferencial de OB y SO. Tinción β-catenina nuclear sugiere fuertemente OB, mientras que la tinción membranosa / citoplásmica de β-catenina sugiere OS.
Osteoblastoma (OB) and osteosarcoma (OS) are 2 bone tumors that predominantly affect young adults. The clinical management of OS differs significantly from that of OB, and thus, accurate diagnosis of OB and OS is critical in determining appropriate treatment modality. However, in certain cases, OS significantly overlaps with OB in clinical and radiographic characteristics, and therefore, the differential diagnosis of OB and OS can be difficult, especially when biopsy material is insufficient. To date, there have been few reports on markers for differential diagnosis of OB and OS. We have previously shown that the Wnt/β-catenin pathway is inactivated in OS. In this study, we aimed to investigate whether the cellular distribution pattern of β-catenin is a potential marker for the differential diagnosis of OB and OS. Immunohistochemical staining was studied in 17 OB samples (21 biopsies; 17 primary and 4 recurrent) and 37 OS samples with complete follow-up information. Moderate-to-strong nuclear β-catenin staining was found in all OB specimens (17/17). In contrast, positive staining of β-catenin was found in the cytoplasm and/or membrane but not the nucleus in all 32 cases of nonchondroblastic OS (32/32) and the classic OS component in chondroblastic OS (5/5). The only positive nuclear β-catenin staining detected in OS biopsies was in chondroblastic OS cells (5/5). In summary, our results indicate that, in addition to conventional histopathologic evaluation, cellular distribution of β-catenin may be used as a valuable marker in the differential diagnosis of OB and OS. Nuclear β-catenin staining strongly suggests OB, whereas cytoplasmic/membranous staining of β-catenin suggests OS.
β-catenin is a valuable marker for differential diagnosis of osteoblastoma and osteosarcoma
Received: September 25, 2013; Received in revised form: February 16, 2014; Accepted: February 28, 2014; Published Online: March 12, 2014
Summary
Osteoblastoma (OB) and osteosarcoma (OS) are 2 bone tumors that predominantly affect young adults. The clinical management of OS differs significantly from that of OB, and thus, accurate diagnosis of OB and OS is critical in determining appropriate treatment modality. However, in certain cases, OS significantly overlaps with OB in clinical and radiographic characteristics, and therefore, the differential diagnosis of OB and OS can be difficult, especially when biopsy material is insufficient. To date, there have been few reports on markers for differential diagnosis of OB and OS. We have previously shown that the Wnt/β-catenin pathway is inactivated in OS. In this study, we aimed to investigate whether the cellular distribution pattern of β-catenin is a potential marker for the differential diagnosis of OB and OS. Immunohistochemical staining was studied in 17 OB samples (21 biopsies; 17 primary and 4 recurrent) and 37 OS samples with complete follow-up information. Moderate-to-strong nuclear β-catenin staining was found in all OB specimens (17/17). In contrast, positive staining of β-catenin was found in the cytoplasm and/or membrane but not the nucleus in all 32 cases of nonchondroblastic OS (32/32) and the classic OS component in chondroblastic OS (5/5). The only positive nuclear β-catenin staining detected in OS biopsies was in chondroblastic OS cells (5/5). In summary, our results indicate that, in addition to conventional histopathologic evaluation, cellular distribution of β-catenin may be used as a valuable marker in the differential diagnosis of OB and OS. Nuclear β-catenin staining strongly suggests OB, whereas cytoplasmic/membranous staining of β-catenin suggests OS.
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